Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

Blog Article

Summary: A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to here an aliasing effect owing to limited effective voxel-sampling rate.In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed.We further provide a free version of our custom acquisition software to assist with a edgewater shoes smooth and easy construction process.For complete details on the use and execution of this protocol, please refer to Borah et al.(2021).